Peptide Testing Hub: COAs, HPLC, Mass Spectrometry and Lot Traceability
Published · Last updated · Publisher: ENOS Labs USA
What does peptide testing show?
Research peptide testing answers three questions about one lot: is it the right molecule (mass spectrometry), how much of it is that molecule (HPLC purity), and can the vial be traced to that record (lot number, laboratory and date). These guides explain each measurement and how to read the documents that report them.
How to Read a Certificate of Analysis (COA) for a Research Peptide
Field by field guide to a research peptide certificate of analysis: identity by mass spectrometry, purity by HPLC, lot number, test date and net peptide content.
HPLC Explained: What High Performance Liquid Chromatography Measures in Peptides
What reverse phase HPLC measures in a peptide sample, how area percent purity is calculated, what the chromatogram shows and what HPLC cannot tell you.
Mass Spectrometry Explained: How Peptide Identity Is Confirmed
How mass spectrometry confirms a research peptide's identity, what ESI and MALDI are, how to read an observed versus expected mass, and the limits of the method.
Purity vs Identity: Why a Peptide Lot Needs Both HPLC and Mass Spectrometry
Purity and identity are separate questions. HPLC measures relative purity, mass spectrometry confirms the molecule. Why each fails alone and what pairing them shows.
Lot Traceability for Research Peptides: Linking a Vial to Its Test Record
What lot traceability means for research peptides, which identifiers should connect a vial to its certificate, and how to record lots in a laboratory notebook.
Research Peptide Quality: Impurities, Content and Stability
The measurable attributes behind research peptide quality: related impurities, net peptide content, counterions, water, aggregation and storage stability.
How to Evaluate a Research Peptide Supplier
A documentation first way to evaluate research peptide suppliers: lot specific COAs, method details, identity testing, traceability and clear research use terms.
To continue, see the peptide testing glossary, the printable supplier checklist and our testing transparency report.
References
Each source below was opened and checked against its PubMed, ClinicalTrials.gov or PubChem record. Links open in a new tab.
- HPLC analysis and purification of peptides (PMID 18604941)Methods in Molecular Biology, 2007 · PubMed
- Liquid Chromatography-High Resolution Mass Spectrometry for Peptide Drug Quality Control (PMID 25716148)The AAPS Journal, 2015 · PubMed
- Related impurities in peptide medicines (PMID 25044089)Journal of Pharmaceutical and Biomedical Analysis, 2014 · PubMed
- Mass spectrometry for protein and peptide characterisation (PMID 11497236)Cellular and Molecular Life Sciences, 2001 · PubMed
These products are sold strictly for in-vitro laboratory research and are not intended for human consumption, diagnosis, treatment, or any therapeutic use. Purchaser must be 18 years of age or older and confirms they are a qualified researcher.